Course Summary
Chemical Biology is an interdisciplinary field that employs chemical techniques to study biological systems. This course will explore the fundamental concepts of Chemical Biology, from small molecules to complex biological systems.Key Learning Points
- Understand the fundamental concepts of Chemical Biology
- Learn how chemical techniques are used to study biological systems
- Explore the potential of Chemical Biology in the development of new drugs and therapies
Job Positions & Salaries of people who have taken this course might have
- USA: $72,000
- India: INR 7,00,000
- Spain: €45,000
- USA: $72,000
- India: INR 7,00,000
- Spain: €45,000
- USA: $95,000
- India: INR 9,50,000
- Spain: €60,000
- USA: $72,000
- India: INR 7,00,000
- Spain: €45,000
- USA: $95,000
- India: INR 9,50,000
- Spain: €60,000
- USA: $85,000
- India: INR 8,50,000
- Spain: €55,000
Related Topics for further study
Learning Outcomes
- Understand the principles of Chemical Biology
- Apply chemical techniques to study biological systems
- Develop an appreciation for the interdisciplinary nature of Chemical Biology
Prerequisites or good to have knowledge before taking this course
- Basic knowledge of chemistry and biology
- Familiarity with scientific research methods
Course Difficulty Level
IntermediateCourse Format
- Online Self-Paced
- Video Lectures
- Quizzes and Assignments
Similar Courses
- Biochemistry: Basics and Beyond
- Introduction to Molecular Biology
Related Education Paths
Notable People in This Field
- Dr. Jennifer Doudna
- Dr. Stuart Schreiber
Related Books
Description
Chemical biology is a burgeoning field that has rapidly risen to prominence. This surge of interest has been fuelled by chemical biology’s applicability to understanding critical processes in live cells or model organisms in real time. This success has arisen because chemical biology straddles a nexus between chemistry, biology, and physics. Thus, chemical biology can harness rapid chemistry to observe or perturb biological processes, that are in turn reported using physical assays, all in an otherwise unperturbed living entity.
Knowledge
- Foundational comprehension of chemical biology
- Interfacial science problem solving and method design
- Understanding of applied, translational concepts in life sciences
Outline
- Welcome to Chemical Biology: Concepts and techniques
- Trailer
- Welcome
- Why study chemical biology?
- Course outline and highlights
- The building blocks: monomeric units of proteins
- The building blocks: monomeric units of DNA and membranes
- Messengers and messages: posttranslational modifications, and pathways
- Studying cellular processes with chemical biology in real time
- Course Acknowledgements
- How assessments work
- The foundation: the cell
- The workhorses: proteins, enzymes
- Classical protein readouts
- Flash mob: overview of protein analysis methods
- Flash mob - Chemistry
- Components of a cell
- Amino acids
- Match technique to goal
- Final Quiz Number 1
- The Devil is in the Minutest Detail
- Welcome to Module 2
- Light'em up! Fluorophores: structures, and fluorescent principles
- Get out (almost) what you put in!
- Traditional light microscopy – applications
- Traditional light microscopy – precautions
- Physics of fluorescence
- Fluorescent proteins
- RNA interference and genome editing
- Plasmids and transfection
- DNA double stranded breaks and γ-H2AX
- Jablonski diagram
- Match color of fluorophore to structure
- Final Quiz Number 2
- A ruler over time and space! Fluorescent assays to measure complex parameters in real time
- Welcome to Module 3
- Creating modified chromatin
- PRC2, a DNA binding complex linked to development and cancer
- TIRF talk
- On our TIRF
- If your protein surfs on DNA, hit the TIRF to study it
- PHF1 Prolongs the stable PRC2-DNA complex, promoting activity
- Fluid mosaic model
- Don’t get uptight! New methods to measure membrane tension
- Getting a grip on membrane tension measurements
- TORCing TORC: the many faces of the TOR proteins
- TORCing it out: pathways that seek to sense and restore membrane tension
- DNA organization in eukaryotes is linked to regulation
- Kinetics of chemical reactions
- Comparing chemical structures
- Flash mob inteins
- Model Organisms
- Making proteins via expressed protein ligation and alkylation
- Hit the tirf: applications of TIRF microscopy
- Analyzing data from TIRF experiments to gain mechanistic insights
- Modern fluorescence methods, the spark of creativity
- TORCing about Tor's role in membrane tension regulation
- Final Quiz 3
- Putting proteins to work for us
- Welcome to Module 4
- Tracking a specific protein in live cells & in real time
- Molecular zipcodes
- How to design your fusion protein
- Design principle and optimization of SNIFITs
- SNIFITs to ID neurotransmitters and drugs
- Application to point of care diagnosis
- Biological electrophiles, the cell’s way of saying “I’m stressed”?
- Breaking the tether is not that bad
- T-REX: a question of control(s)
- T-REX rewiring of kinase activity through targeting AKT and PTEN: opposing forces meet
- Many enzyme mechanisms proceed through a covalent enzyme intermediate that can be stabilized through mutagenesis
- Small molecule signals
- Weighing up our options
- Optimal considerations for making fusion proteins and employing them in chemical biology
- Optimal considerations for making fusion proteins and employing them in chemical biology2
- A bit on the nose? Chose the right SNIFITs
- Final Quiz Number 4
- Making light work of it on a larger and larger scale
- Welcome to Module 5
- A fat lot of use? Proteins that bind and transport lipids
- Greasing the wheels of discovery for new interactomes
- Laser guided lipids
- Deuterium labeling to assess how sphingosine originating in lysosomes is metabolized
- Deuterium labeling and knockout combined
- Mitochondria-released sphingosine does not elicit calcium signaling: global sphingosine release does
- G-REX – a method to release specific electrophiles on demand
- G-REX identifies important novel signaling proteins that function in fish
- Sphingolipids
- Endogenous electrophile signaling
- To cage or not to cage?
- interpretation of pulse chase data from wild type and knockdown lines
- Variables G-REX controls that traditional methods do not
- Final Quiz Number 5
- Casting wide your net
- Welcome to Module 6
- Peptide Nucleic Acid – Use in library synthesis and split and mix approach
- In the lab - split and mix approach in a nutshell
- PNA libraries show their MITE
- It takes two: library diversification through DNA display
- A STING operation: inhibiting a protein implicated in numerous inflammatory diseases
- Performing the Screen
- Mutagenesis: ensuring we stay on target
- The more, the merrier: Phage display, necessity, strengths and weaknesses
- Workflow for split and mix
- Different methods to achieve diversity
- components and controls for Luciferase assay
- Final Quiz Number 6
Summary of User Reviews
Chemical Biology is a highly-rated course on Coursera that explores the intersection of chemistry and biology. Users praise the engaging content and knowledgeable instructor.Key Aspect Users Liked About This Course
The instructor is knowledgeable and presents the material in an engaging way.Pros from User Reviews
- Engaging content
- Useful for both beginners and advanced learners
- Well-organized materials
- High-quality video lectures
- Interactive assignments
Cons from User Reviews
- Some users found the course challenging
- The pace may be too fast for some learners
- Not enough depth in some topics
- Some technical difficulties with the platform
- Limited interaction with other students
Robbie Loewith
4.8 Raiting
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